These higher doses of MSE
Using Kratom For Methadone Withdrawal
also substantially increased cell death within 24 hr (Fig. Using Kratom For Methadone Withdrawal as with the other of cell lines this inhibition of proliferation was accompanied by a dose-dependent increased cell death (Fig. M MIT Using Kratom For Methadone Withdrawal (Table 2. The estimated IC50 values of these cells at 24 hr treatment were 91. Vehicle treated control 0.

A teaspoon of dried leaf is usually between 1. Please note that the dose charts below are for very low potency kratom leaf and leaf powders that are no longer commonly sold in 2014. Every individual reacts differently to every chemical.

Upon resuscitation (as described in chapter 2 section 2. CM0) which was prepared as the normal growth complete media (CM10) but without HIDHS. C (5% CO2).

These recent insights give new perspectives on how cell death may be differentiated and the oncosis term is now more accepted such as in the imperial indo kratom work by Park et al (2000) which showed that the majority of bone marrow-derived mast cells undergo oncosis after IL-3 deprivation kratom therapy free samples (IL-3 have been shown in other studies to be an apoptotic inducer) and only at the later stage showed some apoptotic features (refer to fig. The illustration of morphology of apoptosis and necrosis as originally described by Kerr et al (1972). This diagram was taken from Cruchten and Broeck (2002). Recent illustration of morphology of apoptosis oncosis and necrosis as described by Majno and Joris (1995). Apoptosis pathways Apoptosis is a mechanism by which cells undergo death in response to damage including DNA damage or to control cell proliferation (Ghobrial et al 2005). Various kratom extract uei stimuli can Using Kratom For Methadone Withdrawal trigger apoptosis

and activate two principle signalling pathways namely extrinsic or cytoplasmic pathway and intrinsic or mitochondrial pathways (Ashkenazi 2002; Ghobrial et al 2005). The final execution of apoptosis through these pathways is linked and converges to a common pathway by activating a series of proteases called caspases.

Annexin V conjugate assay for apoptosis detection 5. A possible role of caspases

in MSE and MIT induced cell death 5. Possible involvement of pro-apoptotic caspases (8 and 9) 5. Possible involvement of caspases executor (3 and 7) 5. ROS generation in SH-SY5Y cells treated with MSE and MIT 5.

Many commercial kits tailored to detect several important caspases such as Caspase 3 7 8 and 9 are readily available and most of them can either be analysed via flow cytometry fluorescence or even absorbancemeasurement. The assessment of p53 levels and its target gene p21 which are highly associated kratom opm pills with apoptotic Using Kratom For Methadone Withdrawal cell death can also be investigated using many in vitro approach such as immunoblotting kratom green tea (Western blot) fluorescence image cytometry etc (Mckenzie et al 1999). The generation of ROS in mediating the cell death should also be a major concern in investigating the in vitro assessment of cell death as ROS is a major indicator for mitochondrial dysfunction which in turn could activate many forms of programmed cell death (Tan et al 1998) and a common method to measure the ROS generation in live cells is using the 27-dichlorofluorescein dye (DCFH) (Esposti 2002). Justification Objectives and Hypothesis 1. Justification The use of Mitragyna speciosa Korth or kratom leaves is now popular among traditional users and drug addicts in Using Kratom For Methadone Withdrawal Southeast Asia mainly in Malaysia best opiate detox method and Thailand. With no legislation against possession in other countries apart from the source countries including Australia kratom leaves are becoming popular for selftreatment and as an aid for opiate withdrawal treatment and furthermore the numerous vendors selling this plant over internet has made it widely available to people around the Using Kratom For Methadone Withdrawal globe.