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Thus following DNA damage during initiation stage the cell undergoes mutations which induce more proliferation but not differentiation. Rapidly dividing cells have less time for DNA to get repaired and to remove the DNA-adducts (covalent binding of chemicals with DNA) (Richardson et al 1986; Frowein 2000) and these cells may remain latent over time (Player et al 2004) until the next stage promotion. This second stage starts when promoter influences increase the cell proliferation in susceptible tissues increases the genetic changes and also the cell growth control (Mehta 1995 Oliveira et al 2007).

MIT was determined. A standard curve was

generated using synthetically pure MIT from which the MIT content in MSE fractions Kali Green Indo Kratom was estimated. D-NMR analyses of MSE and MIT

of the different sources (Malaysia and Japan) wee performed using 1H-NMR 400 Mhz spectrometer (Bruker). MSE sample was dissolved in absolute ethanol and centrifuged at 1000 r.

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